The 2012 RUFORUM Biennial Conference is the third in the series. The main objective of the Biennial conferences is to provide a platform for agricultural research for development stakeholders in Africa and beyond to actively exchange findings and experiences, while at the same time learning lessons towards improving performance of the agricultural sector and ultimately people’s livelihoods. The biennial conference is RUFORUM’s most comprehensive meeting for the diversity of stakeholers in agriculture. It is especially dedicated to graduate students and their supervisors, grantees in RUFORUM member universities and alumni. It is a platform for peer review, quality control, mentorship, networking and shared learning. The third Biennial Conference was attended by 657 participants.This record contains an extended abstract accepted under the theme of Crop Improvement
High Frequency Adventitious Root and Shoot Regeneration in Recalcitrant Sweet Potato Cultivars
Abstract:
resistance to weevils through conventional hybridisation has
been limited by the lack of resistance sources in the sweetpotato
germplasm. The aim of this study was to investigate induction
of adventitious shoots which would facilitate genetic
transformation of I. batatas with weevil resistance genes.
Media with different concentrations of thidiazuron alone or in
combination with an auxin were used to investigate induction
of adventitious shoots from whole leaves and stem internode
segments of two I. batatas cultivars, Kyebandula and Bwanjule.
Shoot regeneration was successfully achieved from the two
explants of both cultivars. Statistical analysis showed that type
of explant effect was significant (P < 0.05) for regenerating
shoots. The results of this study are important for I. batatas
breeding This is particularly important for African cultivars which
have hitherto been difficult to regenerate in vitro.
L’amélioration des spécificités de la patate douce (Ipomoea
batatas) comme la résistance aux charançons par hybridation
conventionnelle a été limitée par le manque de sources de
résistance dans le matériel génétique de la patate douce. Le
but de cette étude était d’étudier l’induction de pousses
adventives qui faciliteraient la transformation génétique des I.
batatas avec des gènes de résistance du charançon. Les médias
avec différentes concentrations de thidiazuron seul ou en
combinaison avec une auxine ont été utilisés pour étudier
l’induction de pousses adventives à partir de feuilles entières et
d’endiguer les segments internodaux de deux cultivars de I.
batatas, les Kyebandula et Bwanjule. La régénération des
pousses a été réalisée avec succès à partir de deux explants de
ces deux cultivars. L’analyse statistique a montré que le typed’effet d’explant a été significatif (P <0,05) pour la régénération
des pousses. Les résultats de cette étude sont importants pour
la reproduction de I. batatas. Ceci est particulièrement important
pour les cultivars africains qui ont été jusqu’ici difficiles à
régénérer in vitro.
Language:
English
Extended abstracts submitted under Crop Improvement
Date of publication:
2012
Collection:
RUFORUM Conferences and Workshops
Agris Subject Categories:
Agrovoc terms:
Additional keywords:
Licence conditions:
Open Access
Access restriction:
Form:
Printed resource
Publisher:
Notes:
