Establishment of a Rapid in Vitro System for Regeneration of Papaya Plantlets in a Liquid Media

This study was an attempt to determine rapid micro- propagation methods for regeneration of papaya plantlets in vitro using liquid media. Different concentrations and combinations of growth regulators in liquid MS namely BAP (0-2 mgl-1) and NAA (0-1 mgl-1), CPPU (0-2 mgl-1), and IBA (0-3 mgl-1) were used in the experiments. Results showed that 0.2 mgl-1 BAP, 0.5mgl CPPU were best in shoot multiplication, while 3.0 mg l-1 of IBA in root initiation. Shoot proliferation, root induction reported in this study is an indicator that liquid medium could be a rapid method for papaya regeneration. Further experiments geared towards optimisation of shoot elongation and rooting are ongoing.
Cette étude était une tentative pour déterminer des méthodes rapides de micro-propagation pour la régénération de plantules de papaye in vitro en utilisant des milieux liquides. Différentes concentrations et combinaisons de régulateurs de croissance à l’état liquide de MS à savoir, BAP (0-2 mgl-1) et NAA (0-1 mgl-1), CPPU (0-2 mgl-1), et IBA (0-3 mgl-1 ) ont été utilisées dans les expériences. Les résultats ont montré que 0,2 mgl-1 de BAP, 0.5 mgl-1 de CPPU étaient les meilleures dans la multiplication des pousses, tandis que 3,0 mgl-1 de l’IBA dans l’initiation des racines. La prolifération des pousses et l’induction des racines, rapportées dans cette étude, sont un indicateur que le milieu liquide peut être une méthode rapide pour la régénération de la papaye. D’autres expériences orientées vers l’optimisation de l’allongement des pousses et l’enracinement sont en cours.
Extended abstracts submitted under Crop Improvement
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East Africa
RUFORUM Conferences and Workshops
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The 2012 RUFORUM Biennial Conference is the third in the series. The main objective of the Biennial conferences is to provide a platform for agricultural research for development stakeholders in Africa and beyond to actively exchange findings and experiences, while at the same time learning lessons towards improving performance of the agricultural sector and ultimately people’s livelihoods. The biennial conference is RUFORUM’s most comprehensive meeting for the diversity of stakeholers in agriculture. It is especially dedicated to graduate students and their supervisors, grantees in RUFORUM member universities and alumni. It is a platform for peer review, quality control, mentorship, networking and shared learning. The third Biennial Conference was attended by 657 participants.This record contains an extended abstract accepted under the theme of Crop Improvement