"The 2010 RUFORUM Biennial Conference was the second in the series. The main objective of the Biennial conferences is to provide a platform for agricultural research for development stakeholders in Africa and beyond to actively exchange findings and experiences, while at the same time learning lessons towards improving performance of the agricultural sector and ultimately people’s livelihoods. The biennial conference is RUFORUM’s most comprehensive meeting for the diversity of stakeholers in agriculture. It is especially dedicated to graduate students and their supervisors, grantees in RUFORUM member universities and alumni. It is a platform for peer review, quality control, mentorship, networking and shared learning. This record contains an extended abstract accepted under the theme of agronomy".
Evaluation of micro and macro propagation techniques of Gerbera (Gerbera Jamesonii) under different conditions
Abstract:
Gerbera has gained popularity in the last year in many countries
of the world and it is in great demand in the floral industry as
cut flower as well as potted due to its beauty, colour, long vase
life, and ability to dehydrate after long transportation.
Conventional propagate is too slow. Accordingly, the current
investigation was undertaken with the objectives of establishing
the most suitable micropropagation techniques of Gerbera in
Sudan and to select the best medium/media for
macropropagation of Gerbera. Shoot tips and petioles of five
Gerbera cultivars were tested for shoot and root induction. Three
kinds of cytokinins, BAP, Kintin and 2ip, were used for induction,
and two auxins, IBA and NAA were used for root induction at
four concentrations of each. Different growth media, including
different ratios of peatmoss, sand and silt, were used in
macropropagation experiment. For shoot induction, BAP was
found the best since it resulted in the highest number of shoots/
plantlet. Moreover, 1mg/l concentration was the best of BAP.
For root induction, the two auxins, IBA and NAA, gave
comparable results, with NAA being slightly better up to 1 mg/
l. Among the growth media used in in-vivo propagation peat
moss was the best medium for propagation.
Le Gerbera a gagné la popularité durant la dernière année dans
de nombreux pays du monde et il est en grande demande dans
l’industrie florale comme fleur coupée aussi bien que fleur en
pot grâce à sa beauté, sa couleur, sa longue vie en vase et sa
capacité de déshydratation après un long transport. Une
propagation conventionnelle est aussi lente. En conséquence,
la présente recherche a été entreprise avec comme objectifs
d’établir les techniques les plus appropriées de micropropagation
du Gerbera au Soudan et de sélectionner le meilleur milieu / milieux pour la macropropagation de Gerbera. Les bouts de
pousse et les petioles de cinq cultivars de Gerbera ont été testés
pour l’induction de pousse et de racine. Trois types de cytokines,
BAP, Kintin et 2iP ont été utilisés pour l’induction, et deux
auxines, IBA et NAA ont été utilisées pour l’induction de la
racine à quatre concentrations chacune. Les différents milieux
de croissance, y compris les différentes proportions de mousse
de tourbe, de sable et de limon, ont été utilisés dans l’expérience
de macropropagation. Pour l’induction de pousse, BAP a été
trouvé le meilleur car il a entraîné le plus grand nombre de
pousses / plantule. En outre, la concentration d’1 mg / l était la
meilleure de la BAP. Pour l’induction des racines, les deux
auxines, IBA et NAA, ont donné des résultats comparables,
avec l’ANA étant un peu mieux jusqu’à 1 mg / l. Parmi les
milieux de croissance utilisés in-vivo la mousse de propagation
de tourbe est le meilleur milieu de propagation.
Language:
English
Date of publication:
2010
Country:
Region Focus:
East Africa
University/affiliation:
Collection:
RUFORUM Conferences and Workshops
Agris Subject Categories:
Agrovoc terms:
Additional keywords:
Licence conditions:
Open Access
Access restriction:
Form:
Printed resource
Publisher:
ISSN:
E_ISSN:
Edition:
Extent:
121-125
Notes:
