Maize streak virus (MSV) diversity in Uganda and the assessment of gene silencing as a tool for development of resistance to MSV

Abstract: 
Through an extensive survey conducted in all maize-growing regions in Uganda as well as the application of gene silencing to develop a novel disease control option for maize streak disease, the diversity of Maize streak virus (MSV) in different regions in the country as well as a new disease resistance source were established. Sequence data indicated that all Ugandan MSV isolates belong to the maize-infecting MSV-A1 subtype that is predominant in many sampled areas in East Africa and that both inter- and intra-subtype recombination is common in Ugandan MSVs. Using a transient assay, it was established that MSV replication was abolished or greatly inhibited by a hairpin of a 678-bp portion of an intronless MSV REP gene. The rep hairpin construct also significantly reduced replication of three southern African isolates as well as Ugandan isolates identified in this study.
Grâce à une vaste enquête menée dans toutes les régions de culture du maïs en Ouganda ainsi que l’application de l’inactivation génique pour développer une option nouvelle de contrôle de la maladie pour la maladie de la striure du maïs, la diversité des virus de la striure du maïs (MSV) dans différentes régions du pays ainsi que une nouvelle source de résistance à la maladie ont été établies. Les données séquentielles ont indiqué que tous les isolats ougandais de MSV appartiennent au soustype du MSV-A1 infectant le maïs qui est prédominant dans de nombreux secteurs échantillonnés en Afrique de l’Est et que la recombinaison des sous-types inter et intra est commune dans le virus de la striure de maïs ougandais. En utilisant une analyse transitoire, il a été établi que la réplication du MSV a été abolie ou fortement inhibée par une épingle à cheveux d’une portion de 678-bp d’un gène de REP MSV sans intron. La complexité de l’’épingle à cheveux a également réduit de façon significativela réplication de trois isolats de l’Afrique australe ainsi que les isolats de l’Ouganda ont été identifiés dans cette étude.
Language: 
Extended abstracts submitted under Crop Improvement
Date of publication: 
2012
Country: 
Region Focus: 
Southern Africa
Collection: 
RUFORUM Conferences and Workshops
Licence conditions: 
Open Access
Access restriction: 
Form: 
Printed resource
Publisher: 
Notes: 

 
The 2012 RUFORUM Biennial Conference is the third in the series. The main objective of the Biennial conferences is to provide a platform for agricultural research for development stakeholders in Africa and beyond to actively exchange findings and experiences, while at the same time learning lessons towards improving performance of the agricultural sector and ultimately people’s livelihoods. The biennial conference is RUFORUM’s most comprehensive meeting for the diversity of stakeholers in agriculture. It is especially dedicated to graduate students and their supervisors, grantees in RUFORUM member universities and alumni. It is a platform for peer review, quality control, mentorship, networking and shared learning. The third Biennial Conference was attended by 657 participants.This record contains an extended abstract accepted under the theme of Crop Improvement