Mass propagation of selected papaya planting materials through shoot tip culture in Kenya

Abstract: 
The objective of this study was to optimise media conditions in order to develop an efficient plantlet regeneration system of papaya planting materials. Twelve combinations of different levels of BAP (0.1, 0.3, 0.5 & 0.7mg/L) and NAA (0.05, 0.1 & 0.2mg/L) were used for shoot regeneration. The highest rate of shoot multiplication was recorded in 0.5mg/L BAP combined with 0.1 mg/L NAA. On the other hand, 0.1mg/L BAP combined with 0.05mg/L NAA produced the longest shoots in the three local papaya varieties. 2.5mg/l IBA induced about 85% of the shoots to root. Thus, an efficient plantlet regeneration system for Kenyan papaya through shoot tip culture was successfully developed.
L’objectif de cette étude était d’optimiser les conditions du milieu afin de développer un système efficace de régénération des plantules de matériel de plantage de papaye. Douze combinaisons de différents niveaux de BAP (0,1 ; 0,3 ; 0,5 et 0,7 mg / L) et NAA (0,05 ; 0,1 et 0,2 mg / L) ont été utilisées pour la régénération des pousses. Le plus haut taux de multiplication des pousses a été enregistré en 0,5 mg / L de BAP associé avec 0,1 mg / L d’ANA. D’autre part, 0,1 mg / L de BAP associé à 0,05 mg / L d’ANA a produit les plus longues pousses dans les trois variétés locales de papaye. 2.5mg / l de IBA ont induit environ 85% des pousses à la racine. Ainsi, un système efficace de régénération des plantules de la papaye kenyane par la culture des bouts de pousse a été développé avec succès.
Language: 
Extended abstracts submitted under Crop Improvement
Date of publication: 
2012
Country: 
Region Focus: 
East Africa
Collection: 
RUFORUM Conferences and Workshops
Agris Subject Categories: 
Licence conditions: 
Open Access
Access restriction: 
Form: 
Printed resource
Publisher: 
Notes: 

The 2012 RUFORUM Biennial Conference is the third in the series. The main objective of the Biennial conferences is to provide a platform for agricultural research for development stakeholders in Africa and beyond to actively exchange findings and experiences, while at the same time learning lessons towards improving performance of the agricultural sector and ultimately people’s livelihoods. The biennial conference is RUFORUM’s most comprehensive meeting for the diversity of stakeholers in agriculture. It is especially dedicated to graduate students and their supervisors, grantees in RUFORUM member universities and alumni. It is a platform for peer review, quality control, mentorship, networking and shared learning. The third Biennial Conference was attended by 657 participants.This record contains an extended abstract accepted under the theme of Crop Improvement