Potential Application of Natural Variability of Arabidopsis Thaliana Accessions in Genomics of Xanthomonas Campestris pv Pusacearum

Abstract: 
The banana Xanthomonas wilt epidemic in the Great Lake region of Africa has destroyed banana and is affecting livelihoods of thousands of people who depend on the crop. Currently, there are no banana varieties resistant to the disease. Genetic engineering is the most feasible approach to introduce resistance to the disease in banana. This however requires a good understanding of the underlying genomics of the bacterium. In this study, the capacity of Arabidopsis thaliana to study Xanthomonas strain diversity was evaluated. Two isolates of Xanthomonas campestris (Xcm2251, Xcm4383) and one isolate of Xanthomonas vasicola (Xvv206) were infiltrated into four A. thaliana genotypes. There was variable response by A. thaliana accessions towards the three bacterial strains. The effect of Xanthomonas strains was also significant. It is concluded that A. thaliana can be used for host pathogen interaction studies of Xcm and Xvv.
L’épidémie de fanage Xanthomonas de la banane dans la région des Grands Lacs de l’Afrique a détruit la banane et affecte les moyens de subsistance de milliers de personnes qui dépendent de la culture. Actuellement, il n’existe pas de variétés de bananes qui résistent à cette maladie. Le génie génétique est l’approche la plus réaliste pour l’introduction de la résistance à la maladie de la banane. Cela exige toutefois une bonne compréhension de la génomique sous-jacente de la bactérie. Dans cette étude, la capacité d’Arabidopsis thaliana pour étudier la diversité des souches de Xanthomonas a été évaluée. Deux isolats de Xanthomonascampestris (Xcm2251, Xcm4383) et un isolat de Xanthomonasvasicola (Xvv206) ont été infiltrés en 4 génotypes de A. thaliana. Il y avait une réponse variable par les adhésions de A. thaliana vers les trois souches bactériennes. L’effet des souches de Xanthomonas étaitégalement significatif. Il est conclu que A. thaliana peut être utilisé pour des études hôte d’interaction des agents pathogènes de Xcm et Xvv.
Language: 
Extended abstracts submitted under Integrated Crop Management
Date of publication: 
2012
Country: 
Region Focus: 
East Africa
Collection: 
RUFORUM Conferences and Workshops
Agris Subject Categories: 
Licence conditions: 
Open Access
Access restriction: 
Form: 
Printed resource
Publisher: 
Notes: 

The 2012 RUFORUM Biennial Conference is the third in the series. The main objective of the Biennial conferences is to provide a platform for agricultural research for development stakeholders in Africa and beyond to actively exchange findings and experiences, while at the same time learning lessons towards improving performance of the agricultural sector and ultimately people’s livelihoods. The biennial conference is RUFORUM’s most comprehensive meeting for the diversity of stakeholers in agriculture. It is especially dedicated to graduate students and their supervisors, grantees in RUFORUM member universities and alumni. It is a platform for peer review, quality control, mentorship, networking and shared learning. The third Biennial Conference was attended by 657 participants.This record contains an extended abstract accepted under Integrated Crop Management