Detection and screening of yellow and sweet calabash passion fruit for resistance to woodiness disease in Uganda

Abstract: 
In Uganda passion fruit woodiness disease (PWD) damages passion fruit, causing yield losses of 60-100%. This study aimed at detecting and screening yellow and sweet calabash passion fruit for resistance to PWD. Four viruses, namely, Cowpea aphid borne mosaic virus, Cucumber mosaic virus, Passiflora latent virus, and Tomato ringspot virus were detected using ELISA. Only Cowpea Aphid borne mosaic virus showed a weak reactivity with antiserum, indicating its presence in the tested samples in low concentrations. The detected virus was used to mechanically inoculate yellow and sweet calabash to observe symptom development. This is still on-going.
En Ouganda, la maladie de passiflore (PWD) endommage ce fruit, causant des pertes de rendement de 60-100%. Cette étude a visé à détecter et examiner la passiflore jaune et sucrée du calebassier pour la résistance à la maladie PWD. Quatre virus, à savoir, Cowpea aphid borne mosaic virus, Cucumber mosaic virus, Passiflora latent virus et Tomato ringspot virus ont été détectés, employant ELISA. Seulement Cowpea Aphid borne mosaic virus a montré une réactivité faible avec l’antisérum, indiquant sa présence dans les échantillons examinés dans de faibles concentrations. Le virus détecté a été employé pour inoculer mécaniquement le calebassier jaune et sucré afin d’observer le développement de symptôme. Ceci est encore en cours.
Language: 
Date of publication: 
2010
Country: 
Region Focus: 
East Africa
University/affiliation: 
Collection: 
RUFORUM Conferences and Workshops
Licence conditions: 
Open Access
Access restriction: 
Form: 
Printed resource
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ISSN: 
E_ISSN: 
Edition: 
Extent: 
247-250
Notes: 

"The 2010 RUFORUM Biennial Conference was the second in the series. The main objective of the Biennial conferences is to provide a platform for agricultural research for development stakeholders in Africa and beyond to actively exchange findings and experiences, while at the same time learning lessons towards improving performance of the agricultural sector and ultimately people’s livelihoods. The biennial conference is RUFORUM’s most comprehensive meeting for the diversity of stakeholers in agriculture. It is especially dedicated to graduate students and their supervisors, grantees in RUFORUM member universities and alumni. It is a platform for peer review, quality control, mentorship, networking and shared learning. This record contains an extended abstract accepted under the theme diseases".