Efficient regeneration and transformation systems for improving resistance to weevils in Ugandan sweetpotato cultivars

Sweetpotato is cultivated worldwide as a valuable source of food. Weevils cause 60-100% loss of productivity. The present study aims at genetically transforming selected Ugandan sweetpotato cultivars with genes that confer resistance to weevils. A tissue culture method is being optimised to be coupled with Agrobacterium transformation technique for regeneration of transgenic sweetpotato. Preliminary results show ability to form embryogenic callus in six tested cultivars. Magabali cultivar has shown best response of 72% embryogenic callus. Plant genotype, plant organ and concentration of growth hormone in media have shown significant effect on somatic embryogenesis and could affect plant regeneration efficiency.
La patate douce est cultivée dans le monde entier comme source adéquate de nourriture. Les charançons causent la perte de productivité pouvant s’étendre de 60% à 100%. La présente étude vise à transformer génétiquement les cultivars sélectionnés de patate douce ougandais avec les gènes qui transmettent la résistance aux charançons. Une méthode de culture de tissu est optimisée pour être couplée à la technique de transformation d’Agrobacterium pour la régénération de la patate douce transgénique. Les résultats préliminaires montrent la capacité de former le calus embryogénique dans six cultivars examinés. Le cultivar de Magabali a montré la meilleure réponse du calus embryogénique de 72%. Le génotype de la plante, l’organe végétal et la concentration de l’hormone de croissance dans les médias ont montré l’effet significatif sur l’embryogenèse somatique et pourraient affecter l’efficacité de régénération de la plante.
Date of publication: 
Region Focus: 
East Africa
RUFORUM Conferences and Workshops
Licence conditions: 
Open Access
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Printed resource

About the RUFORUM 2010 Biennal Conference
"The 2010 RUFORUM Biennial Conference was the second in the series. The main objective of the Biennial conferences is to provide a platform for agricultural research for development stakeholders in Africa and beyond to actively exchange findings and experiences, while at the same time learning lessons towards improving performance of the agricultural sector and ultimately people’s livelihoods. The biennial conference is RUFORUM’s most comprehensive meeting for the diversity of stakeholders in agriculture. It is especially dedicated to graduate students and their supervisors, grantees in RUFORUM member universities and alumni. It is a platform for peer review, quality control, mentorship, networking and shared learning. This record contains an extended abstract accepted under the theme of Plant Breeding"