Enhancing Callus induction and embrogenic cell suspension development in East African highland banana

Improvement of banana using conventional breeding methods is very difficult due to its biology. Genetic transformation is a novel alternative approach for crop improvement. Transformation of EA-AAA bananas has, however, been limited by difficulties in callus induction and embryogenic cell suspension (ECS) development. The existing protocols are not readily responsive. In this study N-phenyl-N’-1,2,3-thidiazol-5ylurea (TDZ) and N-(2-chloro-4-pyridyl)-N-phenylurea (4CPPU) that are highly active in regulating morphogenesis in the tissue culture of many plant species have been applied to develop embryogenic callus and ECS culture systems with reliable regeneration efficiency to enhance genetic improvement and propagation of EA-AAA bananas. Preliminary observations indicate that most growth regulator combinations have potential for Callus induction.
L’amélioration de la banane employant des méthodes de reproduction conventionnelle est très difficile à cause de sa biologie. La transformation génétique est une approche alternative originale pour l’amélioration de récolte. La transformation des bananes EA-AAA a été cependant limitée par des difficultés dans l’induction de calus et le développement de la suspension de cellules embryogénique (ECS). Les protocoles existants ne sont pas aisément sensibles. Dans cette étude N-phenyl-N’-1,2,3-thidiazol-5-ylurea (TDZ) et N-(2chloro-4-pyridyl)-N-phenylurea (4-CPPU) qui sont très actives dans la morphogenèse de régulation dans la culture de tissu de beaucoup d’espèces de plante ont été appliqués pour développer les systèmes embryogéniques de calus et de culture d’ECS avec l’efficacité fiable de régénération pour augmenter l’amélioration et la propagation génétiques des bananes EA- AAA. Les observations préliminaires indiquent que la plupart des combinaisons de régulateur de croissance ont le pouvoir pour l’induction de calus.
Date of publication: 
Region Focus: 
East Africa
RUFORUM Conferences and Workshops
Licence conditions: 
Open Access
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Printed resource

"The 2010 RUFORUM Biennial Conference was the second in the series. The main objective of the Biennial conferences is to provide a platform for agricultural research for development stakeholders in Africa and beyond to actively exchange findings and experiences, while at the same time learning lessons towards improving performance of the agricultural sector and ultimately people’s livelihoods. The biennial conference is RUFORUM’s most comprehensive meeting for the diversity of stakeholers in agriculture. It is especially dedicated to graduate students and their supervisors, grantees in RUFORUM member universities and alumni. It is a platform for peer review, quality control, mentorship, networking and shared learning. This record contains an extended abstract accepted under the theme plant breeding".