About the RUFORUM 2010 Biennal Conference
"The 2010 RUFORUM Biennial Conference was the second in the series. The main objective of the Biennial conferences is to provide a platform for agricultural research for development stakeholders in Africa and beyond to actively exchange findings and experiences, while at the same time learning lessons towards improving performance of the agricultural sector and ultimately people’s livelihoods. The biennial conference is RUFORUM’s most comprehensive meeting for the diversity of stakeholders in agriculture. It is especially dedicated to graduate students and their supervisors, grantees in RUFORUM member universities and alumni. It is a platform for peer review, quality control, mentorship, networking and shared learning. This record contains an extended abstract accepted under the theme of Plant Breeding"
Efficient regeneration and transformation systems for improving resistance to weevils in Ugandan sweetpotato cultivars
Abstract:
Sweetpotato is cultivated worldwide as a valuable source of
food. Weevils cause 60-100% loss of productivity. The present
study aims at genetically transforming selected Ugandan
sweetpotato cultivars with genes that confer resistance to
weevils. A tissue culture method is being optimised to be coupled
with Agrobacterium transformation technique for regeneration
of transgenic sweetpotato. Preliminary results show ability to
form embryogenic callus in six tested cultivars. Magabali cultivar
has shown best response of 72% embryogenic callus. Plant
genotype, plant organ and concentration of growth hormone in
media have shown significant effect on somatic embryogenesis
and could affect plant regeneration efficiency.
La patate douce est cultivée dans le monde entier comme
source adéquate de nourriture. Les charançons causent la perte
de productivité pouvant s’étendre de 60% à 100%. La présente
étude vise à transformer génétiquement les cultivars
sélectionnés de patate douce ougandais avec les gènes qui
transmettent la résistance aux charançons. Une méthode de
culture de tissu est optimisée pour être couplée à la technique
de transformation d’Agrobacterium pour la régénération de la
patate douce transgénique. Les résultats préliminaires montrent
la capacité de former le calus embryogénique dans six cultivars
examinés. Le cultivar de Magabali a montré la meilleure réponse
du calus embryogénique de 72%. Le génotype de la plante,
l’organe végétal et la concentration de l’hormone de croissance
dans les médias ont montré l’effet significatif sur
l’embryogenèse somatique et pourraient affecter l’efficacité
de régénération de la plante.
Language:
English
Date of publication:
2010
Country:
Region Focus:
East Africa
University/affiliation:
Collection:
RUFORUM Conferences and Workshops
Agrovoc terms:
Additional keywords:
Licence conditions:
Open Access
Access restriction:
Form:
Printed resource
Publisher:
ISSN:
E_ISSN:
Edition:
Extent:
525-529
Notes:
