"The 2010 RUFORUM Biennial Conference was the second in the series. The main objective of the Biennial conferences is to provide a platform for agricultural research for development stakeholders in Africa and beyond to actively exchange findings and experiences, while at the same time learning lessons towards improving performance of the agricultural sector and ultimately people’s livelihoods. The biennial conference is RUFORUM’s most comprehensive meeting for the diversity of stakeholers in agriculture. It is especially dedicated to graduate students and their supervisors, grantees in RUFORUM member universities and alumni. It is a platform for peer review, quality control, mentorship, networking and shared learning. This record contains an extended abstract accepted under the theme plant breeding".
Enhancing Callus induction and embrogenic cell suspension development in East African highland banana
Abstract:
Improvement of banana using conventional breeding methods
is very difficult due to its biology. Genetic transformation is a
novel alternative approach for crop improvement.
Transformation of EA-AAA bananas has, however, been
limited by difficulties in callus induction and embryogenic cell
suspension (ECS) development. The existing protocols are not
readily responsive. In this study N-phenyl-N’-1,2,3-thidiazol-5ylurea
(TDZ) and N-(2-chloro-4-pyridyl)-N-phenylurea (4CPPU)
that are highly active in regulating morphogenesis in
the tissue culture of many plant species have been applied to
develop embryogenic callus and ECS culture systems with
reliable regeneration efficiency to enhance genetic improvement
and propagation of EA-AAA bananas. Preliminary observations
indicate that most growth regulator combinations have potential
for Callus induction.
L’amélioration de la banane employant des méthodes de
reproduction conventionnelle est très difficile à cause de sa
biologie. La transformation génétique est une approche
alternative originale pour l’amélioration de récolte. La
transformation des bananes EA-AAA a été cependant limitée
par des difficultés dans l’induction de calus et le développement
de la suspension de cellules embryogénique (ECS). Les
protocoles existants ne sont pas aisément sensibles. Dans cette
étude N-phenyl-N’-1,2,3-thidiazol-5-ylurea (TDZ) et N-(2chloro-4-pyridyl)-N-phenylurea
(4-CPPU) qui sont très actives
dans la morphogenèse de régulation dans la culture de tissu de
beaucoup d’espèces de plante ont été appliqués pour développer
les systèmes embryogéniques de calus et de culture d’ECS
avec l’efficacité fiable de régénération pour augmenter
l’amélioration et la propagation génétiques des bananes EA- AAA. Les observations préliminaires indiquent que la plupart
des combinaisons de régulateur de croissance ont le pouvoir
pour l’induction de calus.
Language:
English
Date of publication:
2010
Country:
Region Focus:
East Africa
University/affiliation:
Collection:
RUFORUM Conferences and Workshops
Agris Subject Categories:
Agrovoc terms:
Additional keywords:
Licence conditions:
Open Access
Access restriction:
Form:
Printed resource
Publisher:
ISSN:
E_ISSN:
Edition:
Extent:
405-408
Notes:
