The 2012 RUFORUM Biennial Conference is the third in the series. The main objective of the Biennial conferences is to provide a platform for agricultural research for development stakeholders in Africa and beyond to actively exchange findings and experiences, while at the same time learning lessons towards improving performance of the agricultural sector and ultimately people’s livelihoods. The biennial conference is RUFORUM’s most comprehensive meeting for the diversity of stakeholers in agriculture. It is especially dedicated to graduate students and their supervisors, grantees in RUFORUM member universities and alumni. It is a platform for peer review, quality control, mentorship, networking and shared learning. The third Biennial Conference was attended by 657 participants.This record contains an extended abstract accepted under the theme of Crop Improvement
Establishment of a Rapid in Vitro System for Regeneration of Papaya Plantlets in a Liquid Media
Abstract:
This study was an attempt to determine rapid micro- propagation
methods for regeneration of papaya plantlets in vitro using liquid
media. Different concentrations and combinations of growth
regulators in liquid MS namely BAP (0-2 mgl-1) and NAA (0-1
mgl-1), CPPU (0-2 mgl-1), and IBA (0-3 mgl-1) were used in the
experiments. Results showed that 0.2 mgl-1 BAP, 0.5mgl CPPU
were best in shoot multiplication, while 3.0 mg l-1 of IBA in root
initiation. Shoot proliferation, root induction reported in this study
is an indicator that liquid medium could be a rapid method for
papaya regeneration. Further experiments geared towards
optimisation of shoot elongation and rooting are ongoing.
Cette étude était une tentative pour déterminer des méthodes
rapides de micro-propagation pour la régénération de plantules
de papaye in vitro en utilisant des milieux liquides. Différentes
concentrations et combinaisons de régulateurs de croissance à
l’état liquide de MS à savoir, BAP (0-2 mgl-1) et NAA (0-1
mgl-1), CPPU (0-2 mgl-1), et IBA (0-3 mgl-1 ) ont été utilisées
dans les expériences. Les résultats ont montré que 0,2 mgl-1 de
BAP, 0.5 mgl-1 de CPPU étaient les meilleures dans la
multiplication des pousses, tandis que 3,0 mgl-1 de l’IBA dans
l’initiation des racines. La prolifération des pousses et l’induction
des racines, rapportées dans cette étude, sont un indicateur
que le milieu liquide peut être une méthode rapide pour la
régénération de la papaye. D’autres expériences orientées vers
l’optimisation de l’allongement des pousses et l’enracinement
sont en cours.
Language:
English
Extended abstracts submitted under Crop Improvement
Date of publication:
2012
Country:
Region Focus:
East Africa
Collection:
RUFORUM Conferences and Workshops
Agris Subject Categories:
Agrovoc terms:
Additional keywords:
Licence conditions:
Open Access
Access restriction:
Form:
Printed resource
Publisher:
Notes:
