Cassava brown streak disease (CBSD) is emerging as one of the most important viral diseases of cassava (Manihotesculenta) and is considered today as the biggest threat to cassava cultivation in East Africa. The disease is caused by isolates of at least two phylogenetically distinct species of single-stranded RNA viruses belonging to the family Potyviridae, genus Ipomovirus. The two species are present predominantly in the coastal lowland [Cassava brown streak virus (CBSV);Tanzania and Mozambique] and highland [Cassava brown streak Uganda virus (CBSUV);Lake Victoria Basin,Uganda,Kenya and Malawi] in East Africa. In this study, we demonstrate that CBSD can be efﬁciently controlled using RNA interference (RNAi).Three RNAi constructs targeting the highland species were generated, consisting of the full-length (FL; 894 nucleotides), 397-nucleotide N-terminal and 491nucleotide C-terminal portions of the coat protein (CP) gene of a Ugandan isolate of CBSUV (CBSUV-[UG:Nam:04]), and expressed constitutively in Nicotiana benthamiana. After challenge with CBSUV-[UG:Nam:04], plants homozygous for FL-CP showed the highest resistance, followed by the N-terminal and C-terminal lines with similar resistance.In the case of FL,approximately 85% ofthetransgenicplantlinesproducedwerecompletelyresistant. Some transgenic lines were also challenged with six distinct isolates representing both species:CBSV and CBSUV.In addition to nearly complete resistance to the homologous virus, two FL plant lines showed 100% resistance and two C-terminal lines expressed 50–100% resistance, whereas the N-terminal lines succumbed to the nonhomologous CBSV isolates.Northern blotting revealed a positive correlation between the level of transgene-speciﬁc small interfering RNAs detected in transgenic plants and the level of virus resistance.Thisistheﬁrstdemonstration of RNAi-mediated resistance to CBSD and protection across very distant isolates (more than 25% in nucleotide sequence) belonging to two different species: Cassava brown streak virus and Cassava brown streak Uganda virus.
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RUFORUM Journal Articles